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Contents:
  1. Clinical trials for Haemophilus Influenzae Type B
  2. Haemophilus Influenzae | Wisconsin Department of Health Services
  3. Recommended for you

Federal Government. Read our disclaimer for details. Results First Posted : August 21, Last Update Posted : September 20, Study Description. Haemophilus influenza type b Hib remains a serious global health threat associated with high mortality and morbidity in young children. In China, The overall impact of Hib-related infections and the extent of coverage of Hib conjugate vaccines are unclear. Generally, vaccination has been considered the most effective way to prevent infection against Hib.

In order to evaluate safety and immunogenicity of a haemophilus influenzae type b Hib conjugate vaccine developped by Chengdu Olymvax Biopharmaceuticals Inc.

FDA Resources. Arms and Interventions. Hib conjugate vaccine. Children aged months: 3-dose 0,28,56 d ;Children aged months: 2-dose 0,28 d ;Children aged years:one dose 0 d , 0. Outcome Measures.

Clinical trials for Haemophilus Influenzae Type B

The mutant library can be used in any control and stress condition. Data analysis is in detail described in Langereis et al. This protocol is adapted from a previously published paper: Langereis et al. T able 2. Your questions will be directed to the authors of the protocol. The authors will be requested to answer your questions at their earliest convenience.

Once your questions are answered, you will be informed using the email address that you register with bio-protocol. You are highly recommended to post your data including images for the troubleshooting. My bio page Edit user profile Signature Reset the password. A peer-reviewed protocol journal. No publication fee; no access fee.

Jeroen D. Langereis jeroen. Original research article A brief version of this protocol appeared in:. Similar Protocols. Reproducibility Feedback. Share your feedback.

Abstract The genome-wide screen Tn-seq van Opijnen et al. Keywords : Haemophilus influenzae, Transposon sequencing, Tn-seq Figure 1. Donor DNA Any type of DNA that carries a mariner transposon with MmeI restriction site in the inverted repeat can serve as donor for transposon in the in vitro mariner transposition reaction. Generation of mutant library We provide a detailed protocol for the generation of a mutant library in non-typeable Haemophilus influenzae , but this can be used for all bacteria that are naturally competent.

Part II. Mutant library screen As example, we provide a detailed protocol for the identification of non-typeable Haemophilus genes essential for survival in environmental air, as published before Langereis et al. Remove supernatant with 1 ml pipet do not touch the pellet. Note: At this moment it is not necessary to carefully remove al liquid.

Carefully remove all supernatant with a 1 ml pipet do not touch the pellet. Dissolve pellet in Centrifuge the bacteria 10 min with 3, x g and resuspend in 10 ml PBS. Centrifuge the bacteria 10 min with 3, x g and resuspend in 1 ml M-IV medium and transfer to a 1. Note: The number of colonies transposon mutants is dependent on the transformation efficiency of the NTHi strain used in this experiment.

It is recommended to use a highly competent NTHi strain in order to obtain large mutant libraries. Alternatively, multiple transformations can be pooled to obtain sufficient transposon mutants, typically fold the number of genes in the genome. Mutant library screen The mutant library can be used in any control and stress condition.

Haemophilus Influenzae | Wisconsin Department of Health Services

Haemophilus influenzae Protocols. Hood E. Front Matter Pages i-xi.


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Gail G. Hardy, Simone M. Tudor, Joseph W. Geme III. Pages General Methods for Culturing Haemophilus influenzae.

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Transformation of Haemophilus influenzae. Diagnosis of Infection. Characterization of Plasmids.